{"id":256946,"date":"2024-12-10T05:52:06","date_gmt":"2024-12-10T05:52:06","guid":{"rendered":"https:\/\/project.uniurb.it\/vitality\/?p=256946"},"modified":"2025-10-09T14:27:07","modified_gmt":"2025-10-09T14:27:07","slug":"a-biotechnological-approach-for-the-production-of-new-protein-bioplastics","status":"publish","type":"post","link":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/","title":{"rendered":"A biotechnological approach for the production of new protein bioplastics"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”2px|||||” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”50px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font_size=”16px” custom_margin=”||40px||false|false” global_colors_info=”{}”]<\/p>\n

Biotechnol J. 2024 Jan;19(1):e2300363. doi: 10.1002\/biot.202300363. Epub 2023 Oct 13.<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font_size=”16px” custom_margin=”||40px||false|false” global_colors_info=”{}”]<\/p>\n

De Marchis F, Vanzolini T, Maricchiolo E, Bellucci M, Menotta M, Di Mambro T, Aluigi A, Zattoni A,
Roda B, Marassi V, Crinelli R, Pompa A.<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” locked=”on” global_colors_info=”{}”]<\/p>\n

Abstract<\/h3>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

The future of biomaterial production will leverage biotechnology based on the domestication of cells as biological factories. Plants, algae, and bacteria can produce low-environmental impact biopolymers. Here, two strategies were developed to produce a biopolymer derived from a bioengineered vacuolar storage protein of the common bean (phaseolin; PHSL). The cys-added PHSL* forms linear-structured biopolymers when expressed in the thylakoids of transplastomic tobacco leaves by exploiting the formation of inter-chain disulfide bridges. The same protein without signal peptide (\u0394PHSL*) accumulates in Escherichia coli inclusion bodies as high-molar-mass species polymers that can subsequently be oxidized to form disulfide crosslinking bridges in order to increase the stiffness of the biomaterial, a valid alternative to the use of chemical crosslinkers. The E. coli cells produced 300 times more engineered PHSL, measured as percentage of total soluble proteins, than transplastomic tobacco plants. Moreover, the thiol groups of cysteine allow the site-specific PEGylation of \u0394PHSL*, which is a desirable functionality in the design of a protein-based drug carrier. In conclusion, \u0394PHSL* expressed in E. coli has the potential to become an innovative biopolymer.<\/span><\/p>\n

\u00a9 2023 The Authors. Biotechnology Journal published by Wiley-VCH GmbH.
DOI: 10.1002\/biot.202300363
PMID: 37801630 [Indexed for MEDLINE]<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||0px|||” locked=”on” global_colors_info=”{}”]<\/p>\n

Keyword<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

Biopolymer, disulfide bridges, E. coli, phaseolin, transplastomic plants<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||0px|||” locked=”on” global_colors_info=”{}”]<\/p>\n

Link<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” link_option_url_new_window=”on” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

https:\/\/hdl.handle.net\/11576\/2723351<\/a>
https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.202300363<\/a><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Biotechnol J. 2024 Jan;19(1):e2300363. doi: 10.1002\/biot.202300363. Epub 2023 Oct 13.De Marchis F, Vanzolini T, Maricchiolo E, Bellucci M, Menotta M, Di Mambro T, Aluigi A, Zattoni A,Roda B, Marassi V, Crinelli R, Pompa A.AbstractThe future of biomaterial production will leverage biotechnology based on the domestication of cells as biological factories. Plants, algae, and bacteria can […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

Abstract<\/span><\/h3>

The future of biomaterial production will leverage biotechnology based on the domestication of cells as biological factories. Plants, algae, and bacteria can produce low-environmental impact biopolymers. Here, two strategies were developed to produce a biopolymer derived from a bioengineered vacuolar storage protein of the common bean (phaseolin; PHSL). The cys-added PHSL* forms linear-structured biopolymers when expressed in the thylakoids of transplastomic tobacco leaves by exploiting the formation of inter-chain disulfide bridges. The same protein without signal peptide (\u0394PHSL*) accumulates in Escherichia coli inclusion bodies as high-molar-mass species polymers that can subsequently be oxidized to form disulfide crosslinking bridges in order to increase the stiffness of the biomaterial, a valid alternative to the use of chemical crosslinkers. The E. coli cells produced 300 times more engineered PHSL, measured as percentage of total soluble proteins, than transplastomic tobacco plants. Moreover, the thiol groups of cysteine allow the site-specific PEGylation of \u0394PHSL*, which is a desirable functionality in the design of a protein-based drug carrier. In conclusion, \u0394PHSL* expressed in E. coli has the potential to become an innovative biopolymer.<\/span><\/p>

Person<\/h3>

Dott.ssa Francesca De Marchis<\/p>

Keyword<\/h3>

biopolymer, disulfide bridges, E. coli, phaseolin, transplastomic plants<\/span><\/p>

Link<\/h3>

For more information: https:\/\/ora.uniurb.it\/item\/preview.htm?uuid=ecd59baa-c280-41cb-89e3-2014813bafad<\/a><\/p>

\u00a0<\/p>

\u00a0<\/p>","_et_gb_content_width":"","footnotes":""},"categories":[20],"tags":[43,44,45,46,47],"class_list":["post-256946","post","type-post","status-publish","format-standard","hentry","category-wp2","tag-biopolymer","tag-disulfide-bridges","tag-e-coli","tag-phaseolin","tag-transplastomic-plants"],"yoast_head":"\nA biotechnological approach for the production of new protein bioplastics - Vitality<\/title>\n<meta name=\"description\" content=\"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A biotechnological approach for the production of new protein bioplastics - Vitality\" \/>\n<meta property=\"og:description\" content=\"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\" \/>\n<meta property=\"og:site_name\" content=\"Vitality\" \/>\n<meta property=\"article:published_time\" content=\"2024-12-10T05:52:06+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-10-09T14:27:07+00:00\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Scritto da\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minuti\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/15b1057b2f252eccd39e050ca6322397\"},\"headline\":\"A biotechnological approach for the production of new protein bioplastics\",\"datePublished\":\"2024-12-10T05:52:06+00:00\",\"dateModified\":\"2025-10-09T14:27:07+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\"},\"wordCount\":459,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#organization\"},\"keywords\":[\"Biopolymer\",\"disulfide bridges\",\"E. coli\",\"phaseolin\",\"transplastomic plants\"],\"articleSection\":[\"WP2\"],\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\",\"url\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\",\"name\":\"A biotechnological approach for the production of new protein bioplastics - Vitality\",\"isPartOf\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#website\"},\"datePublished\":\"2024-12-10T05:52:06+00:00\",\"dateModified\":\"2025-10-09T14:27:07+00:00\",\"description\":\"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.\",\"breadcrumb\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/project.uniurb.it\/vitality\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"A biotechnological approach for the production of new protein bioplastics\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#website\",\"url\":\"https:\/\/project.uniurb.it\/vitality\/\",\"name\":\"Vitality\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/project.uniurb.it\/vitality\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"it-IT\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#organization\",\"name\":\"Vitality\",\"url\":\"https:\/\/project.uniurb.it\/vitality\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/project.uniurb.it\/vitality\/wp-content\/uploads\/2023\/05\/vitality-pnrr-loghi.004.png\",\"contentUrl\":\"https:\/\/project.uniurb.it\/vitality\/wp-content\/uploads\/2023\/05\/vitality-pnrr-loghi.004.png\",\"width\":500,\"height\":200,\"caption\":\"Vitality\"},\"image\":{\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#\/schema\/logo\/image\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/15b1057b2f252eccd39e050ca6322397\",\"name\":\"admin\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/8fbfff81b839d10c731939719cc9258934e1e2486b05052c44f9d6ba0147b5fa?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/8fbfff81b839d10c731939719cc9258934e1e2486b05052c44f9d6ba0147b5fa?s=96&d=mm&r=g\",\"caption\":\"admin\"},\"sameAs\":[\"https:\/\/project.uniurb.it\/vitality\"],\"url\":\"https:\/\/project.uniurb.it\/vitality\/author\/donatellotrisolino_nojzyyu8\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"A biotechnological approach for the production of new protein bioplastics - Vitality","description":"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/","og_locale":"it_IT","og_type":"article","og_title":"A biotechnological approach for the production of new protein bioplastics - Vitality","og_description":"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.","og_url":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/","og_site_name":"Vitality","article_published_time":"2024-12-10T05:52:06+00:00","article_modified_time":"2025-10-09T14:27:07+00:00","author":"admin","twitter_card":"summary_large_image","twitter_misc":{"Scritto da":"admin","Tempo di lettura stimato":"2 minuti"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#article","isPartOf":{"@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/"},"author":{"name":"admin","@id":"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/15b1057b2f252eccd39e050ca6322397"},"headline":"A biotechnological approach for the production of new protein bioplastics","datePublished":"2024-12-10T05:52:06+00:00","dateModified":"2025-10-09T14:27:07+00:00","mainEntityOfPage":{"@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/"},"wordCount":459,"commentCount":0,"publisher":{"@id":"https:\/\/project.uniurb.it\/vitality\/#organization"},"keywords":["Biopolymer","disulfide bridges","E. coli","phaseolin","transplastomic plants"],"articleSection":["WP2"],"inLanguage":"it-IT","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/","url":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/","name":"A biotechnological approach for the production of new protein bioplastics - Vitality","isPartOf":{"@id":"https:\/\/project.uniurb.it\/vitality\/#website"},"datePublished":"2024-12-10T05:52:06+00:00","dateModified":"2025-10-09T14:27:07+00:00","description":"Engineering phaseolin protein into bioplastics via E. coli and tobacco systems: high yield, disulfide crosslinking, and PEGylation potential.","breadcrumb":{"@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#breadcrumb"},"inLanguage":"it-IT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/project.uniurb.it\/vitality\/a-biotechnological-approach-for-the-production-of-new-protein-bioplastics\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/project.uniurb.it\/vitality\/"},{"@type":"ListItem","position":2,"name":"A biotechnological approach for the production of new protein bioplastics"}]},{"@type":"WebSite","@id":"https:\/\/project.uniurb.it\/vitality\/#website","url":"https:\/\/project.uniurb.it\/vitality\/","name":"Vitality","description":"","publisher":{"@id":"https:\/\/project.uniurb.it\/vitality\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/project.uniurb.it\/vitality\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"it-IT"},{"@type":"Organization","@id":"https:\/\/project.uniurb.it\/vitality\/#organization","name":"Vitality","url":"https:\/\/project.uniurb.it\/vitality\/","logo":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/project.uniurb.it\/vitality\/#\/schema\/logo\/image\/","url":"https:\/\/project.uniurb.it\/vitality\/wp-content\/uploads\/2023\/05\/vitality-pnrr-loghi.004.png","contentUrl":"https:\/\/project.uniurb.it\/vitality\/wp-content\/uploads\/2023\/05\/vitality-pnrr-loghi.004.png","width":500,"height":200,"caption":"Vitality"},"image":{"@id":"https:\/\/project.uniurb.it\/vitality\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/15b1057b2f252eccd39e050ca6322397","name":"admin","image":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/project.uniurb.it\/vitality\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/8fbfff81b839d10c731939719cc9258934e1e2486b05052c44f9d6ba0147b5fa?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8fbfff81b839d10c731939719cc9258934e1e2486b05052c44f9d6ba0147b5fa?s=96&d=mm&r=g","caption":"admin"},"sameAs":["https:\/\/project.uniurb.it\/vitality"],"url":"https:\/\/project.uniurb.it\/vitality\/author\/donatellotrisolino_nojzyyu8\/"}]}},"_links":{"self":[{"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/posts\/256946","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/comments?post=256946"}],"version-history":[{"count":14,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/posts\/256946\/revisions"}],"predecessor-version":[{"id":257904,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/posts\/256946\/revisions\/257904"}],"wp:attachment":[{"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/media?parent=256946"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/categories?post=256946"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/project.uniurb.it\/vitality\/wp-json\/wp\/v2\/tags?post=256946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}